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especially if they clean up afterward by licking their own crotch
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We've all done it: Gulped down a glass of water, swirled some hot water around in the cup, and placed it in the dish drainer. Or used our kitchen scissors for a quick snip of a meat package, dipped them under running water, and called it a day. But anything coming in contact with your mouth or with raw meat carries bacteria and needs to be washed in hot, soapy water.
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All bone marrow grafts were collected, treated with mafosfamide, washed, and cryopreserved. They were then thawed at 42 in a water bath and immediately infused into the patient's central line without further manipulation. The marrow cell suspension was depleted of RBCs and neutrophils using a buffy coat and a Ficoll (FM) gradient centrifugation method as described by Gilmore et al.23 The final cell suspension was washed twice using minimal essential medium (S MEM), supplemented with ABO compatible human plasma (50%), and adjusted to a concentration of 2 107 WBC Mafosfamide was added at a median dose of 70 g 107 WBC (range, 60 according to the individual sensitivity of granulocyte colony forming units, as described previously.24 The marrow suspension was incubated with agitation at 37 for 30 min. To terminate the cytotoxic effect of mafosfamide, the cell canada goose jackets vancouver suspension was washed once at 4 resuspended with ABO compatible human plasma to a final volume of 200 ml, and transferred to four freezing bags (DELMED, Canton, MA, USA), 50 ml per bag. Fifty milliliters of cold S MEM supplemented with 20% DMSO was added to each bag. The cell suspension was then immediately frozen at a controlled rate of 1 to 2 per min from 10 to 70 and at 7 per min to 100 (BV 10 Biological Freezer; Cryoson, Krefeld, Germany).
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